Plus Two Biology: Inheritance, Biotechnology, Human Health | Exam Winner

[00:00:08]hello good evening biotechnology the principles organism and population then evolution okay bad gamer bad gamer bad gamer yes goodies shakar lovely Soul girl drowning Legend very good amus G Shri okay principles of inheritance human health and diseases then biotechology principles okay [Music] yes good evening okay yes okay for okay ready okay okay ready okay yes okay to 2 21 yes very good keep it up biology biology principles basic principle okay okay very good com en D techology rest enence loation very good very good seic sequences palic sequences what g a t t c g a a t t c 5 sequence it is a sequence of DNA sequence of DNA which read same same which read same on both strand when reading reading frame kept same reading frame okay when reading frame kept samec sequence very good and poic sequences palindromic sequences are sequences of DNA which read same on both strand when reading frame kept same again important okay of organism and second second two letters species species name species name okay okay ah yes strain of bacteria strain of bacteria stra and Roman letter number in which the order in which it is isolated order in which the en okay yes R3 okay ready restriction en name the two types of restriction enzyme restriction enzymes are of two types excle ucle okay [Music] EX yes specific loation name the two kinds of restriction enzymes restriction how simple exonucleases endonucleases Exon nucleus and ucle enm explain the naming procedure of restriction enzyme with one example genus genus of species genus first first letter of species okay okay stra of bacteria strain of bacteria r y13 r y13 the order in which it is isolated order in which isolated it is isolated sequences recogn sequence restriction enzymes correct location palic sequence it is a specific DNA sequence which read the same on both the strands when the reading reading frame is same 5 D to das frame seen same okay yes question what are palindromic DNA nucleotide sequences write one example for palic sequences term sequences terms sequence of DNA sequence Ofna onand when reading frame is same when read reading frame kept same reading frame for example five Das Das five Das 3 Das g a a t t c g a a t t c 5 Dash 3 Dash set very good okay the nule sequence name this kind of nucleotide sequence sequences palic sequences ready palindromic sequences Define and DNA sequence uh which read the same on both the strands when reading frame is kept the same okay vectors origin of replication sequence of DNA sequence of DNA from where from where replication start replication start replication start okay then selectable markers mark to identify transformant transformant from non- transformant transform mark yes non [Music] transformance for cling yes okay marking s s artificial PBR 322 very good PBR 322 PBR 322 okay okay PBR 3 322 marker orig replication origin replication replication of plas replication of plasmid okay ready yes okay resist [Music] resistance anbi an tety resistance okay okay okay pst1 P1 E1 CL one hind 3 B Sal one1 P2 PST Hind yes hey cloning site very good PBR [Music] 3 [Music] for for [Music] okay okay and [Music] okay [Music] 322cl resistance sequ okay an okay resistance resistance okay a [Music] okay is a sequence of DNA where replication originate replication and any piece of DNA when linked to this sequence can be made to replicate okay selectable marker selectable marker PBR 322 resisty resistance normal okay addition to orig of replication the vector PBR 322 require selectable marker selectable marker which helps in identifying and eliminating you are out okay okay so form is a procedure through transim the Gen encoding resistance to antibiotic mark inactivation insertional inactivation biology okay chromogenic subst en no full silence ch for chromogenic trans insertion ination okay ready PBR 322r 322 write three characteristic features of cloning Vector clone [Music] Mar okay observe the cloning vector and explain the following yes orig of replication orig replication or of replication it is a sequence of DNA from where replication originate okay it is a sequence of DNA sequence of DNA from where from where replication originate replication originate sequence replication originate ready1 very good there is a specific sequence with responsible for initiating replication very Gooding singing C for cloning cite for restriction enzyme bom H1 restriction enzyme enzyme identify the missing feature so simple term 2 2017 very good mhamad yes orite yes okay for examplei t to transform normal plant cells into tumor and direct these tumor cells to produce chemics okay TI plasmid the tumor inducing TI plasmid of AGR bacterian tumas has now been modified into a which is no more pathogenic PL animal rrus disarm one of the important feature of cloning Vector is the presence of selectable markering vector write the role of selectable marker name the selectable markers used in PBR 322 selectable markers in markers in selectable markers [Laughter] yes and to identify to identify to identify transformance from non- transformance transformance okay write other two features vectors [Music] full Mark yes full mark okay during genetic engineering Vector with a foreign DNA is transferred into host bacteria okay the next Target will be the selection selection how antibiotic resistance and insertional inactivation exploited for this purpose anotic resistance ins antibiotic resistance selectively permitting the growth of transform non transformance will not survive in in the case of transformance case of transformance they have they have antibiotic resistance transformance antibiotic resistance antibiotic resistance and they will survive they will survive in a medium containing in a medium containing antibiotic antibiotic canot survive non- transformant cannot survive non- transformant cannot survive because because they don't have don't have because they don't have genes for resistance genes for antibiotic resistance and transform okay yes selectively permitting the growth of transformance only ins inactivation insertional inactivation insal in in the case of transformance transformance transformance will not form transformance will not form will not form blue color in the presence of in the presence of chromogenic substance because beta galactosides beta galoes galactosides get inac okay non transformance produ non transformance will form color will form blue color yes okay [Music] m inje micro injection micro DNA is directly injected is directly is directly injected directly injected into the nucleus disen okay DNA is coed is coed on gold tungsten micro particle gold or tungsten micro particle it is bombarded it is bombarded with a high velocity with a high velocity High veloc okay PL cell DNA is bombarded with a high velocity of by using micro particles of golden gold and tungsten Co with the DNA okay treating with a treating with a specific divalent C like okay shock treatment heat shock treatment treat disarmed pathogen disarmed pathogen question how can we make a host cell competent to receive a foreign DNA or with treating with d valent calent heat shock [Music] treatment ready okay biolis micro injection ready CLE okay ready okay J read in a biotechnology book that alien DNA can be introduced into host cell by micro injection and biolistics micro injection in animal cell animal C in animal animal cell micro injection DNA is directly injected directly injected into the nucleus into the nucleus inje in cell DNA is bombarded DNA is bombarded with high velocity okay uh micro particles micro particles of go gold or tungsten gold or tungsten Co with gold with DNA with processes of recombinant DNA technology okay d [Music] ah G d prot [Music] isation First Step rest restriction separation of desired fragment electr for very good sh po re streaming millions of copies isolation of gentic material cutting of gentic material cutting and separation okay rest restri en separation separation and isolation ofs okay is negatively charged netiv char electrically already okay very good eum bromide bromium broe color bright bright orange color okay bright orange color bright very good illusion illusion Desir fragment okay bright orange color bands of DNA okay yes illusion okay the DNA fragment purified in this way are used in constructing recombinant DNA na small okay M very good questions the identify the technique electr Forest is so simple so elegant how can you visualize the separated DNA fragment fragments very good eum bromide treating with staining with BR then exposing into then exposing with with UV light bright orange colored bands appear bright orange colored band appear okay yes what [Music] ution DNA is cut out and extracted from the gel piece from electroforesis is a technique to separate fragments of DNA from a mixture some of the events of elect given below 4 3 2 1 St DNA yes 34 21 very good Chain Reaction polymerization biolog okay po po multiple cop primer very good need very good very good Aqua th aquaticus thermos aquaticus thermos aquaticus okay th stable very good small Syle that are complim to the okay S5 D okay exension polymerization bil denaturation and healing extension in the molecular basis in the molecular basis okay in the principles of inheritance human health ready identify the process the process PCR PCR polymerized Chain Reaction write the steps of this process very good denaturation analing extension okay done what is a primer Prim small chemically synthesized okay a small chemically synthesized chemically synthesized SE nucleotides sequence which are complimentary to the DNA which are complimentary to the DNA fragment of sequence yes question PCR stands for polymerized chain reaction what is the function of PCR in recombinant DNA technology very good yeah ready amplification then name the three steps denaturation denaturation analing then extension done any name the DNA polymerize enzyme used in PCR and the bacterium from which it is isolated enzyme are very good T polymerase T polymerase bacteria name the orm from which it is isolated th aquaticus ther aquaticus done ready okay question in recombinant DNA technology amplification of genus done by PCR expand PCR PCR Chain Reaction polymerase chain reaction what are the three steps and healing and extension then multiple copies of gene of interest can be synthesized name the technique and it requirements yes very good requ requirements very good copy of gen template DNA template D very good enle name theable enzy used in PC very very very important question Vector okay Po denaturation analing and extension are the three steps of a process used in gene amplification name the process process small chemically synthesized nucleotide that are complimentary to the DNA very good process PCR name the organism from which the DNA polymer enzyme extracted therm aquaticus bacter bacteria bacteria AR bacteria okay yes th aquaticus yes thank you Erin Vincent Yes reactors okay specific products okay bioreactors so raw materials are biologically converted into specific products individual enzyme I prot condition okaya simple T bioreactor simple T [Music] bioreactor down streaming processing down streaming processing separ andf okay formulation with a suitable okay purification and separation biology okay techology is a complex process which involves several steps write down the major steps of recombinant DNA technology okay isolation and separation isolation of isolation and separation of DNA separation and isolation DNA C cutting using restriction enzyme and Separation by gel electroforesis amplification of desired fragment amplification PC Desir fragment insert introduce into the introduce recombinant DNA into the host cell host cell host ctru obtaining theu product obtaining product last separation and purification obtaining product product separation and purification down strring [Music] processing okay question observe the relationship of the first pair and fill in the blank restriction enzyme cutting of DNA joining of DNA the Restriction enzyme cutting of DNA joining very good theny the instrument biology principl human health and diseases and principles of inheritance ah simple s tank okay simple sred tank bioreactor okay write any one use of this instrument instrument use to biological product to obtain product product okay yes simple s Tang bioreactor name the instrument used to produce large quantity of recombinant protein recant protein Syle bioreactor PCR cloning Vector PC yes cloning Vector PBR 322 very good PCR Tac ah PCR Tac polymerase very good P A5 A5 then three separation fragments in the separation of fragments separation of DNA fragments c c C2 okay C2 G separation of fragments 2 three very good electr fores then convert raw material into product raw materials d d yes bioreactor foreign product product then question and this is the last question okay restriction ucle isolated ucle which is isolated very good hind two yes hind two ready hind two okay principles of inheritance okay thank you so much okay chapter clear principles of inheritance human okay thank you so much human health and principles of inheritance okay okay yes yes hello everyone hello hello hello good evening next so principles ofh and yes good evening good evening [Music] yes hell yes ready ready get yes yes okay [Laughter] yes ah yes ready yeah [Laughter] [Laughter] yes yes Anna jby super class thank you m first WI oh [Music] yes yes yes [Laughter] ready yes [Music] guys [Music] [Laughter] yes [Laughter] okay one one guys [Applause] start conratulations okay ready okay yes principles ofit and of in and so who is the father of gentics who is the father of genetics very good mental experiment super F okay many contrasting traits oppos then next very good easy to large numers ofs super fast yes very good s for P for f for flower St for stem characters [Music] sting [Music] [Music] Dom Domin features yes dominant very good okay ready experiment important monoss yes very good yes very good so [Music] cap Capal factors are occurs as Fair [Laughter] characters okay very good yes very good very good [Music] genetic makeup of an [Music] organal photp externally [Music] f capital T capital T capital T small T capital T small T small T small yes very good okay okay genotypic and phenotypic ratio yes very good so 3al T is capital small T is small small genetic makeup of an organism what is the genotypic and phenotypic ratio for monohybrid cross okay ready ready mon Dom very good sh law of dominance so you are not expressed dominance of Domin of dominance first first but intermediate factors factors do not blend G form sep okay law of segregation Crystal Clear ready so mon law of dominance law of segregation okay the figure given below showing mental experiment using p PL identify the cross very good which are the laws proposed by mental based on this observation okay ah yes very good law of dominance yes very good law of of dominance in aimilar pair of facture one Member One member of the pair is dominant and other is recessive law of dominance okay in a dissimilar pair of factor one is expressed that is called dominant and one is not expressed supressed that is called processive of dominance ofation segregation this based on the do not show blending but factors do not show blending during gamid formation they separate each other the pair of segregate from each other is called law of segregation clear clear okay ready mon hybrid [Music] cross small small y small y wrinkled wrinkled green round that is variation sity differ wrinkled green wrinkled round Roundy ratio equal yes 9 3 31 9 3 3 isotypic okay 9 3 9 3 3ot of assment okay y small y small r y y small R small R very good capital r capital y capital r small y small R small small law of independent assortment independent assortment okay dibe test cross and mention it significance describe test okay okay ready T small tal t Okay capital W small W capital W capital vetor flet capital W small W capital W small W capital W small W capital W capital w Capital wal wet small W small okay okay small [Music] dou small W small W white color Flow White [Music] yes small w w small W male female okay W wet very good raet very good small W small w w small w capit w small W cap W small W cap W small wet Viet Violet capital h the ination wowowow det the par parental generation in F1 let you determine fub1 par Generation [Music] generation okay yes very good so question answer clear ready yes clear C okay domination 18 dominance is an example for deviation from mentan inheritance illustrate with example okay menal first deviation incomplete dominance incomplete dominance okay incomplete [Music] dominance [Music] sag okay experiment [Music] yes very good pink color FL small capital r capital r capital r small r capital r small R small R small R so R2 generation genotypic and phenotypic ratio genotypic and yes very good whiteed is pink whiteed is pink 2 to 1 1 is to 2 is one okay phenotypic and genotypic ratio dominance incomplete dominance so so is the phenomena in which phenotype of F1 hybrid offsprings does not resemble any of the parent and the parent is an intermediate between the one incomplete dominance is an example for deviation from M inheritance illustrate with example [Music] examp genot andot synthesis in pant P synthis incomplete Domin example small incomplete dominance okay incomplete dominance set okay ready okay Dev okay examp blood group so BL i a i and small B positive a positive a negative posi okay blood grp small blood gr i i i blood gr a blood grp dominance example blood grp blood gr dominance example AB blood group I and the I and I produce a slightly different form of the sugar while Al does not produce any sugar present together they both Express their own types of sugar this is called Co dominance [Applause] BL yes ready [Music] yes okay okay ready yes if a father is with O blood group and mother is with B blood group write the possible blood groups of their children okay father so I blood gr so first cross I small I small I small I small I so I small then small I then I so blood gr okay chros the of inh [Music] of inheritance yes and sat and B majority mother blood group [Applause] ofh both sperms and eggs contribute equally to theity of [Laughter] sings okay okay yes chromosome carry theit trait [Applause] chromosome the chros pass pass from one generation to next generation without any loss or gain chromosome occurs inros haal the of inh and father of experimental genetics very specious name melanogaster m [Music] simple synthe Med short life ccle they complete their life cycle within two week a single meeting can produce large number of sing of cleation of SE sexualism sexual dimorphism experiment okay yes th Morgan carried out several dihybrid crosses in rosopa mention two reason for selecting drop as an experimental organism Reon re simple synthetic medium they require simple synthetic medium simple synthetic medium easy to cure easy to culture simple synthetic Med SLE mating can produce large numbers of progeny single meeting produce large number of progeny single meeting can produce large number of progeny are clear differentiation of sexes clear differentiation clear differentiation of SE short lifespan they complete their life cycle they complete complete their life cycle within two week within two week okay [Music] dist they are link jeans link jeans best friends h new combin new combin combination new combination okay combination lination l so describe the physical Association of gen on chros L yes so explain the phenomena shown in the following figure and reason differentiate the production of recombinant cross and cross CR b as explained by distance between gen then distance between linkage l okay [Music] combin [Music] experimentals student Al okay measure the distance between gen on a chromos in poic inheritance polygenic iner so many genes control a single trait [Applause] single poic single Gene can exhibit mulp mopy yes okay distinguish between male hamity and female hamity 22 then one 23 of chromosome 22 chos chros chros DET respons chros so chromosome [Music] [Applause] [Music] sex determination okay sex determination examiner sure first one x x x Y type XX X Y Tye x [Music] x okay x x x type of determination X X x0 Type ofation Hopper so grasshopper round worm cockroach XX type of determination very good hom best example on birds birds example type of SE determination very very very mity and female male hetam and female [Music] mity [Applause] [Applause] [Music] famp grasshopper okay then w okay yes ready family that is the family family tree family tree Anis analis it's a diagam of family it's a diagram of family tree record inheritance of certain trait for two or more ancestral generation it is a DI of family tree which record inheritance of certain trait for two or more generation is called pedigree analysis so simp color yes [Music] [Music] me noral Rel meeting between relatives cons meting okay meting affected individuals affected individuals affected individual unfied unpi question answer pedigree study provides a strong tool in human genetics to trace the inheritance of a specific trait Define pedigree analysis pedigree analysis pedigree analysis is the family tree which record inheritance of inheritance in one or two ancestral generation andb so identify the following symbols used in the pedigree analysis so between relativ okay meeting between reles unspecified individual a female very good female disorders and chromosomal disorders dis okay observe the figure given below and answer the question following so first identify the chros the okay [Music] [Music] a for this is aut dominant okay the shed used shent infected individuals affected shaded symbol affected individual shaded simples very good and it is a sex reive father mother father mother then father son then mother daughter so crisscross [Music] relationship so color BL fa to discriminate red or green colored GRE color okay ro royal disase okay Royal disase sex so SLE beding blotting fact absent okay very very very important CLE cell anemia first point it is a autome l recessive disorder autobin chain synthis hob [Music] hemoglobin CH Sy [Music] hog of inhance cell anemia during beta hemoglobin chain synthesis the substitution of glutamic acid by so name thetic disorder Illustrated the okay ready CLE cell anemia RW Theo sequence with normal hemoglobin okay of glutamic acid by Val at the sixth position of hemoglobin chain hemoglobin beta hemoglobin chain synthesis is called [Music] [Music] now very hyrox hyro okay hdro it is an inborn error in metabolism inborn error in metabolism are Menan disorders disorders so what do you mean byan disorder by alteration or mutation in a single Gene single Al m which one of the is an example of metabolism mention the cause of this disorder INB metabolis metab absence of fenale alanin hydroxy phale alanin hydroxy which convert fenal alanin to [Music] thyrosin is aut recessive disorder okay aut recessive disorder chromosome number number sex L recessive disorder that affect the clotting of blood blot [Music] blood hopia [Music] hopia the disorder that is caused by the substitution of glutamic by at the six position of beta hemoglobin chain cell anemia [Applause] metab okay ready okay syndrome down okay okay partially open mouth SLE palmal heart diseases disas [Music] rudimentary ovary rudimentary ovary poly developed okay St yop bre develop identif the and fter Sy okay fter syrr what is the chromosome number in 45 okay ready ready set write any four features of individuals inflicted with t far Tong then partially open mou partially opened mouth then Palm very good Palmas next Point mental retardation conal heart disas congenital heart diseases conal heart disas mental retardation question presence of the additional copy of chromosome 21 was observed in a person during diagnosis identify the genetic disorder so add set of chromosome features of the disorder complete the table using appropriate terms sy4 St F okay some genetic abnormality and their genotype and features are distributed in column A B and C respectively match them correctly match so down Sy syr and 44 yes ready okay okay human health and dises yes human health and okay okay common diseases in [Music] human dis okay high fever weakness loss of appetite tiredness L of headache for disas first one okay first so okay contaminated water and food through contaminated water and food dis [Music] pneumonia streptococus pneumonia so exam hemophilus influen so through drop in breathing yes very good okay okay common group Rus [Music] okay very goodat but not the symptoms n congestion n throat Hess headache tired okay dis first one Malia okay second one constipation abdominal pain and cramps St with excess mu and blood CL yes very good plasmodium VX plasmodium okay mosito okay female anus mosquito then human beings human beings infec INF [Music] sporos and G fation fation [Applause] okay okay name any two protos disease it's cative organism and any two symptoms Malia organ plasmodium plasmodium then Amis disis okay then any two symtoms fever abdominal pain andal pain and with ex which species of parasite cause Mal of fium f can you suggest your method to control malaria mosquito net net use and avoid stagnant water avoid stagnant water and introduce guppy gy fish introduce [Music] symptoms of malaria sexual face gamy male and female g [Music] okay okay through contaminated food and vegetable fruits and water contaminated egg of Paras internal bleeding muscular pain fever blockage of intestinal Passage okay attack so chronic inflammation and deformation of the organs and genital organs okay chronic inflammation and deformation of genetic orans okay sh in the following figure and write thetive organism of the disease okay [Music] whicher area BR of bankr okay micro micro microron and epid micr okay then dry SC Les on the various part of the body SKS so identify the disease and write the scientific names of pathogen from the following symptoms dry scaly lesion on skin nail and scalps followed by intching write the scientific names of the pathogen SCI microron [Music] epidermophyton troyon F okay FAL infection okay yes chronic inflammation of organs and limbs genital organs affected resulting in Gross reformi very good area bankrupty area bankrupty yes ready match the column A with column B and ctive organm and type ofen very good very good one two three ringis very good common C Comm it's a group of viruses called rhus okay observe the list of common observe the list of certain common diseases in human given below and answer the following question common call Malia and identify the bacterial disease among theed identify the bacterial disease bacterial disease very good Mega very then tiredness okay ready question list of some diseases are given below arrange them based on the ctive organism in the table first [Music] Comm common three very good Comm next fisar ascaris FIS amasis any next pneumonia pneumonia innate immunity then aced immunity innate immunity at the of [Music] birth PCC physical barrier physic biological barrier then cellular barrier then last cine barrier PCC okay mucus membrane physical bar tears from the eyesi cellular neut IM P polymorphonuclear lyos macras [Music] very virus infect imity so physical barrier physiological barrier cellular barrier and inate Immunity is characterized by providing different types of barrier name the four types of innate immunity barrier [Music] [Applause] okay and CTO okay physical physiological and cellular and cyto [Music] [Music] barrier following question so type of then phri neutr inter virus infect cell in inter [Music] infity okay yes ready immunity inate immunity so AC immunity and IM [Music] soos okay ready heavy chain heavy chain heavy chain one aning okay different types of imunoglobulin imog different types of antibodies IG G IG g a and IGM IG g e IG [Music] okay day okayer okay observe the figure identify the molecular structure given in the [Music] figure anti name the region as [Music] C antigen binding [Music] site yes okay yes very immunity passive imity IM but long dur long [Music] dur produce its [Music] [Music] antibody [Music] longed prepared antibodies are introduced to the host body body in produced to the host already vaccination vaccination and passive example [Music] okay [Music] next so preparation of antigenic proteins pathogens or inactivated or weaken pathogen are introduced to the body is called what is mean by vac weakened form of weakened or inactivated form of pathogen inactivated form of WR the principle of vaccination memory imate active and passive IM duration longting and prepare its own anination okay [Music] imunity rapid react preformed antium anti IG antibody so IG antibody produce okay rheumatoid arthritis okay IMM second IMM system IMM primary lymphoid organ ime Sy ly or okayyy primary ly or then secondary lymph organ so primary lympho organs B marrow B marrow then thus thus [Music] resp secondary lymph then P patches of small intestine pays patches then lymphatic tissue lymphatic tissue secondary ly very good exp the following lym Ural tract respiratory tract mu Associated lympha tissue okay mucos Associated lym tis human IM deficiency virus human imun deficiency virus hi okay ready deficiency Sy Imo deficiency syndrome syr human IMC singleand materal hi virus single standard hi reverse transcriptase reverse transpal virus deficiency Sy helper sexual contact with infected then through transfusion of contaminated blood and blood products by sharing infected needles as in case of intravenous drug Abus then from infected mother to through fluid okay en PR okay ready so asids has no C prevention awareness cond control organization aess making blood blood banks safe from hi blood free distribution of free distribution of Quant Private Hospital controlling drug abuse regular checkups of for HIV sused population HIV susceptible so expand the term immuno deficiency syndrome acur imuno deficiency Sy then next mention the name of virus that cids HIV virus then name the widely used diag diagnos test en IM then for practices for prevention ofs prevention of aess cond use disposable needles use control drug abuse and free distribution of condom and susceptible population okay uned growth of cells unrolled B tumor Mal tumor B tumor Mal tumor so T just beginning beginning okay spreading of cancer cells okay spreading of cancer C so unrolled unrolled contact inh do not show do not show contact inhibition normal cell contct r toac smoking causes cancer cancer causing canc canc biopsy and biopsy computery MRI scan M okay ready then antibodies against cancer treat biopsy antibodies against canc okay chy biolog response mod okay uh briefly describe the characteristics of cancer do not show contact inhibition contact inhibition do not show cond unrolled C okay yes last topic drug abuse okay first one so gastro Cal Sy okay thenation of Morphin okay different NE hallucination property only temporary happiness okay can Central n system gastro traal system yes very good op [Music] re NE neur then drug WR any for ill effect of drug or alcohol abuse alcol abuse so that's only a temporary Happ Reckless Behavior Reckless Behavior then violence yes very good then lack of personal hygiene yes no to drugs very good Al effect mention any form measure useful for prevention and control of alcohol and Dr drug abuse prevention and [Music] control [Music] UND pressure Avo [Music] undue pure pressure okay yes okay ucation and counseling very good education and counseling education and celing huh education and counseling yes very good seeking seeking Prof yes so avoid undue peer pressure education and counseling seeking help from parents and peers looking for danger and seeking professional and medical help okay already filling the BLS C using correct terms Given In The Box canc cancer passive immunity sensitive to some particle metastasis active immunity autoimmune deficiency immune deficiency disease ah very good Goa metast sity to some partic [Music] Aller respond the exagger respon to IM deficiency syndrome IM [Music] dis okay mother's milk is considered essential for newborn inant name the fluid secreted by mother from the breast during the initial day of lactation our breast very good what type of imunity question of the [Music] day cancer okay Ty IM okay okay what type of immunity type of imity okay hello Mr Mystery okay [Applause] ready of human and ready love you all okay Sher bye-bye love you all T bye-bye