Biotechnology in One Shot: Principles & Processes Class 12 WBCHSE/NEET

[00:00:00]foreign foreign foreign foreign [Applause] [Music] foreign [Applause] foreign um foreign [Applause] [Music] foreign human welfare is foreign biotechnology [Music] okay biotechnology she is [Applause] foreign okay variety of foreign [Music] foreign foreign see hello hybridization process ities European Federation of biotechnology okay European European Federation of biotechnology bio techno logic foreign foreign began foreign foreign bar Equity colon hello foreign [Applause] okay foreign okay old definitions hybridization process hybridization processes foreign foreign foreign foreign okay European Federation of biotechnology is okay um genetic engineering bioprocessing engineering okay genetic engineering bioprocess engineering genetic engineering team connect to chip third Gene DNA is very high protein high protein okay transcription transcription okay [Applause] transcription translation okay foreign [Applause] foreign [Music] [Music] foreign [Applause] docone DNA rashanique cotton of prakriti foreign foreign [Music] foreign foreign AJ I mean uh protein change is I thought according sterile conditions [Applause] sequences [Applause] engineering [Applause] [Music] darling vaccine vaccine protein hormone vaccine genetic engineering processes genetic engineering smart domain hormone vaccine genetic engineering sterile condition maintain Corporation bio process engineering bio process engineering come on Airport okay foreign [Applause] DNA project okay genetic engineering DNA project is [Music] foreign okay Ami would be costly bacteria is DNA DNA Juno prothomi foreign [Music] foreign foreign foreign foreign [Applause] sodium prodisile sulfate okay sodium doticile sulphate Plus [Applause] rnas Devo foreign okay nucleus okay a bar foreign foreign [Music] foreign [Applause] foreign okay air foreign Airport it's a camera is a solution is eternally eternally [Applause] DNA Auto Capital DNA capital okay [Applause] foreign foreign DNA k free talk or we talked a case pulling bowling okay pulling bowling pulling bowling a case pulling bowling is [Applause] a is a solution say Solutions [Applause] restriction endonuclease there are restriction endonuclease Dara DNA cotton dnaire ipshito again [Applause] restrictions restriction endonuclease restriction endonuclease Kachi are no weak intervention important is [Applause] foreign okay a bar restrictions restriction endonuclease foreign [Applause] [Applause] foreign did you hear the Raptor Thomas foreign foreign foreign [Applause] foreign [Applause] okay okay foreign a bacterial DNA is DNA nucleotides foreign [Applause] [Applause] foreign foreign foreign [Applause] foreign foreign doodle new uh sorry okay methylase restriction endonucleus restriction endonuclei okay every restriction foreign Kitty then okay nucleotide okay it is [Applause] okay okay restriction current bacterial viral DNA DNA is okay DNA is foreign foreign [Applause] foreign foreign DNA t methyl group together restriction endonucleaser endonucleaser recognition stunning recognition stunning foreign foreign foreign foreign okay foreign [Music] okay foreign G a t C g t a kind of okay foreign foreign a t 80 nucleotide generation Orton coding okay 8080 nucleotides a t c t a G c a [Music] G a t c g t okay among um t a there is nuclear nucleotide Center sorry nucleus Heights in volume nucleos Heights nucleotide chain nucleotide or endonuclease nucleotide a team nuclear it is foreign foreign restriction endonucleus okay a restriction endonucleus a UTC DNA nucleotide nucleo tight recognition side bowling okay recognition recognition recognition site volume recognition guy is recognition site very recognition site recognition a recognition site a nucleotide nucleo excited from papal indrumikoi palindrome koi okay a recognition site a nucleotide is foreign [Music] sequence palindromic sequence sequence a division importance foreign endonuclease air T is here on hello Eco R1 equals foreign [Applause] proton Borno uchobacteria gone key okay show bacteria namir Borno dto or Treaty of Borno now bacteria genus sorry bacteria species [Music] you need this correct okay okay [Music] foreign [Applause] foreign [Music] true man chunkati named Romance restriction endonucleaser from nidesh Korean sorry foreign among [Applause] one money okay foreign endonuclease dushot bacteria strength show is no bacteria strength okay restrictions foreign particular sequence star identified okay foreign a particular sequence restriction endonuclease Eco R1 in particular sequence foreign foreign foreign okay foreign hello pronto sticky end sticky end cannibals is [Music] foreign [Applause] [Applause] foreign [Applause] [Applause] [Applause] [Applause] foreign [Applause] restriction endonuclease restriction endonucleus DNA nucleotide sequence [Music] foreign Japan egg uh pronto end Ed indicator sticky end o Bob blantoned o lineup restriction endonuclease restriction endonuclease [Music] sticky end Ed party foreign okay AJ sticky end sticky ended nucleotide nucleotide base key board Style by acock okay okay DNA G t c a c g t c g a c is c a g c t g okay Theta 5 Prime to 3 Prime ETA 3 Prime 2 5 Prime okay GTC and G AC and ctg foreign okay foreign okay GTC c a g active g a c t g okay Pi Prime to 3 Prime I prime to 3 Prime 3 Prime 2 5 Prime e Prime to a prime okay it is foreign foreign [Music] chained to okay a hint to chit chalk a hint Ock hello foreign hemophilus influenji Rd audio system take a um next jail electrophoresis DNA DNA DNA okay a DNA r a young so time is foreign okay okay like the restriction endonucleus [Applause] come on foreign sorry flask never ever flasks okay agarose powder agarose powder okay a bar e agarose powdered distilled water Joel Devo amongstable etvr okay ethereum bromide okay foreign foreign [Applause] foreign camera body well recoverably well okay foreign sorry okay at a wheel it avail okay um foreign it works a DNA solution DNA solution so the DNA solution is even back home foreign foreign foreign [Applause] okay videos foreign enzyme the a DNA cartoon DNA foreign all other electrophoresis jail Electro policies okay next [Applause] [Music] next average restriction enzyme say restriction enzyme restriction enzyme foreign [Applause] foreign effect affected agarose gel plated agarose foreign [Applause] positive electrode decay okay positive electrodes photos [Applause] DNA Hollow negative charge Okay negative charge next book now foreign [Applause] so wrong foreign DNA King is [Applause] foreign [Music] ibong borrow DNA to krogly okay borrow DNA to kruguli each one is [Applause] foreign foreign foreign okay next foreign okay DNA jail plated foreign [Applause] actor buffer Nebo actor baffer Nebo sorry sorry foreign okay now DNA um [Applause] foreign foreign chromatography okay column chromatography is foreign okay DNA binding material okay DNA binding material upon DNA binding materials okay Solutions sorry a Solutions DNA binding agent okay DNA binding agent is [Music] okay DNA binding is foreign foreign buffer illusion buffer a illusion buffer delay illusion buffer DNA binding material DNA binding material DNA binding materials okay material [Applause] DNA k free thought coding DNA binding materials electrophoresis [Applause] [Applause] foreign cloning is [Applause] polymerase chain reaction polymerase Chain Reaction polymerase chain reaction movies carry police carry police carry movies foreign amplification amplifications [Applause] denaturation denaturation denaturation okay PTO step the camera will see um primer annealing primer primer annealing first primer songs string call Tom Pro Sharon process started okay denaturation primary primary denaturation denatured who is our teenagers foreign [Applause] foreign [Applause] foreign through John hi foreign for a nucleotide purity nucleotide oligonucleotide primers foreign oligonucleotide nucleotidecty oligonucleotide DNA primer oligo nucleotide vicisto DNA primer foreign DNA string called string call uh duties Duty template between calling events foreign foreign foreign bacteria foreign okay armophilic bacteria armophilic bacteria foreign ophilic bacteria Pharmaceuticals crochet party to bacteria sorry stable Parma stable DNA polymer is enzyme DNA polymerase enzyme foreign College polymerization poly polarization okay DNA string color polymerization is a polymerization foreign foreign TP polymerization Gautier mg2 Plus foreign uh amadeer is polymerization n number of polymerization n times I mean the polymerize two n two to the power n times 2 to the power n chongq sorry 2 to the power n shankock okay foreign 2 to the power n from DNA fine okay now desired DNA Gene Airport vector Vector key vectors [Applause] DNA piece DNA to Chrome jdna Vector DNA first of all Vector ammonia piece of DNA power DNA two group Zeta is foreign [Applause] foreign [Applause] foreign plasmid vector Victor 's DNA Hajj DNA or bacteriophage bacterio pods M13 DNA Hobby cost meat will be back say bacterial artificial chromosome artificial chromosome foreign artificial chromosome East artificial chromosome foreign foreign sorry uh foreign foreign foreign bacteria chromosome okay dto 80 replication a replication a foreign 80 bacteria crochet who classmate Victor okay uh [Music] foreign foreign foreign P22 okay plus meet plasmid T Boulevard foreign this tube number okay okay whether that's a plus medium um victory foreign foreign 80 needhaman near the store man is replication is time taking DNA replication holy AKA origin of replication AKA origin of replication origin of replication okay origin of replication falling is tan Korea okay DNA poppy Shankar need annoying um Ika parlon according to say economics foreign okay multiple cloning sites multiple clothing sites okay applications recognition site present recognition sequence presentation foreign okay sorry uh victory opor Ino even know restriction restriction endonucleaseide recognition site recog nition site or thought only we know restriction enzyme restriction enzyme Vector a Windows okay come on Ebon Amin Jodi chai foreign foreign [Applause] come on among economics foreign cloning site a vectorate multiple cloning site a pupus teeth restriction endonuclease it need this store restriction endonucleaseide nucleoside industrial restriction endonucleoside recognition site k pukto recognition [Applause] recognition engine sorry restriction in restriction enjoying the a portito okay restriction engine DNA foreign [Applause] [Applause] sorry recognition site a foreign foreign [Music] foreign foreign foreign of pontory now big um foreign formation ibala height transformation France torment transformation foreign non-transformant volume is a selectable marker selectable marker non-transformant among transformation selectable marker table marker transformant among non-transformation transformant or non-transformant k on transform and K foreign okay selectable Market transform into non-transformation even non-transforms among transformative okay 80 transformative transformative foreign okay selectable marketing resistance selectable selectable marketing antibody protein sorry antibiotic protein anti piotic antibiotic protein antibiotic protein foreign okay PVR 322.

[03:03:19]foreign engine amprg okay so PST one among PV 18 recognition each one okay Sal one bam each one okay um foreign one now antibiotics Shea bacteria a dudo antibiotics antibiotic shape the antibiotics is foreign [Applause] 322 okay the key Ada PBR 322 PVR 322 I mean PBR 322 PBR 322 bacteria time tetracycline bacteria transformation bacteria transformation foreign foreign if shito please see Victor Plus foreign transformment Volvo vectors not sorry transformation recombinant Volvo sorry sorry recombinant Volvo okay recombinants is foreign foreign foreign foreign foreign foreign [Applause] foreign Ty height is foreign Dean come on yeah Gene okay puc Vector a vector puc vector it is California California University Theta design uh X 3 um okay ibom a lakh z Gene lack Z Gene is [Music] protein synthesis foreign Theta galacto side is okay beta galactositis ebar a H vector k p u c vector k q c vector k w c puc 18.

[03:18:46]foreign transformation transformant transformment E coli so okay growth medium nutrient medium okay um Earth Incorporated about a beta galactose side is okay transformation Theta galacto foreign okay a beta Electro side is a chodium x gal substrate beta electrocidisation X gal up straight d substrate to the ad okay foreign product s foreign okay ibar is Next Step a Ami E coli bacteria puc vector okay E coli bacteria in each e p u c vectorate the Incorporated if shito I mean Incorporated puc vector transformant recombine named okay E coli okay transformment or recombinant E coli Hobby side is [Applause] Gene [Music] together okay beta galactose ideas [Music] porno Hobby okay nutrients they were foreign Dharma day two bacteria bacteria recombinant bacteria now uh recommendables recombinant noise foreign activation insertional inactivations substrate products is AJ [Music] puc vectors solution inactivation [Music] next transformation transformation in the transformation okay transformation okay foreign [Music] foreign okay now okay ice plated okay nice place okay a bacteria okay highlight coaching okay now foreign under cacl2 joke I mean E coli course [Music] test tube k Ube k t borrow put the trade for of 40 prayer of alcohol okay Trader foreign Victor um [Music] foreign foreign E coli air push prachi OTE okay among CA plus iron glow among CA plus iron glow ca2 Plus ion glow ion gulo foreign plus iron ta2 plus iron okay ca2 plus iron ta2 Plus ta2 Plus p a two plus a two plus a c a 2 plus foreign DNA DNA classmate vector plus meet vector port U DNA subscribe positive foreign there were heat shock eat shock okay degree Centigrade 42 degrees Centigrade 42 degree centigrade it is okay sell say shelter is our poor you know 42 degree Centigrade ca2 Plus okay foreign foreign foreign after sale micro injection is micro injection data foreign keep shito okay micro injections foreign [Applause] foreign foreign okay from plant cell relationship ever foreign foreign foreign micro particle Mike Chrome particle jar Ed here in the a Brito sake biggie habitokori prachi foreign okay biologists next three foreign vector agrobacterium tumifications aggro bacterium tumifications agrobacterium tumification bacteria economic bacteria TI plus meat bar tumor produce Curry tumor Kumar utpad on Curry tumor inducing blood splasmid but TI plus means it a j t i plus DNA T DNA almost foreign a bacteria Crown crown crown call Rogue srishti Korea acrobacterium tumifications a agrobacterium bacteria disarmed Curry or thought talking these armed Curry no no hey foreign okay hit three Favorito foreign ers virus retro viruses Tara foreign ers cancer is Uncle virus cancer dividers are there foreign virus k minus K korbo not disarmed Correa these armed Korea transformation foreign next DNA ligase chalk ligation ligation or thought DNA Duty Kata to crore DNA to T 4 [Music] phosphodite first four diastere bandhani Curry okay foreign next bioreactor okay bioreactor key bioreactors large vessels transformed bacteria culture transformed bacteria desired protein if sheet of proteins Electro foreign foreign [Applause] bio reactor Hollow Steelers vessel I bottle vessel foreign shito protein okay is a bioreactor say bioreactor model bio reactor model s see simple steered tank simple steered steered tank bioreactor reactor sparged tank bioreactor okay simple steered tank bioreactor among sparged tank bioreactor your simple steel tank bioreactorable cylindrical shape structure okay editor cylindrical shape structure cylindrical shape structure Passage foreign [Music] bioreactor reactor sterility sterility foreign foreign okay [Music] studies eight uh how many systems Optimum Optimum [Music] temperature Optimum pH oh oxygen level maintain Hobby [Music] foreign [Music] oxygen level foreign flat plated impeller agitator system uh ncrt boy agitator system agitator system agitator system foreign it automated automated a young shooter temperature control temperature control temperatures foreign foreign or should be the Channel is [Applause] bubble so we recorded folly subscribe foreign oxygen mix singing foreign foreign bioreactor bio reactor bubble foreign oxygen transfer a Photoshop oxygen transfer area okay bioreactor systems culture method bioreactor process bioreactor foreign closed system closed system okay we will see continues culture method continues culture I mean thought okay continuous culture method open system open system I mean my channel foreign system a zombie turn on systems continually continuous t-shirts okay or share pipak Jato located you are fine darling so I'm not Igbo J next J topic not Downstream processing Downstream processes foreign foreign foreign ipsito protein got on otar is a amraboli Downstream processing Downstream processing okay hello hello equity actor Steam their resistance foreign trust him come on foreign foreign foreign Tata [Applause]